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rabbit anti at1r  (Alomone Labs)


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    Structured Review

    Alomone Labs rabbit anti at1r
    Rabbit Anti At1r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/at1r/Anti-Angiotensin+II+Receptor+Type-1+(extracellular)+Antibody/pm42248365-88-12-17
    Average 94 stars, based on 53 article reviews
    rabbit anti at1r - by Bioz Stars, 2026-10
    94/100 stars

    Images

    Related Articles

    Triple Immunostaining:

    Article Title: Kinin B1 Receptor Blockade Prevents Angiotensin II-induced Neuroinflammation and Oxidative Stress in Primary Hypothalamic Neurons.
    Article Snippet: For GFAP staining, an Alexa Fluor 488-labeled antibody (#53–9892-82, lot 1,914,350, ThermoFisher, 1:50 dilution) was used. .. Triple immunostaining was performed with specific validated antibodies for detection of AT1R (#AAR-011, lot AN2002, Alomone labs, 1:200 dilution) and B1R (#ABR-011, lot An-01, Alomone labs, 1:200 dilution) coupled with MAP2 and DAPI staining. .. After 3 washes, coverslips were counterstained with DAPI, then mounted with ProLong Diamond (ThermoFisher) antifade medium and examined under a confocal microscope (Zeiss LSM 700).

    Article Title: Kinin B1 Receptor Blockade Prevents Angiotensin II-induced Neuroinflammation and Oxidative Stress in Primary Hypothalamic Neurons
    Article Snippet: For GFAP staining, an Alexa Fluor 488 labeled antibody (#53-9892-82, lot 1,914,350, ThermoFisher, 1:50 dilution) was used. .. Triple immunostaining was performed with specific validated antibodies for detection of AT1R (#AAR-011, lot AN2002, Alomone labs, 1:200 dilution) and B1R (#ABR-011, lot An-01, Alomone labs, 1:200 dilution) coupled with MAP2 and DAPI staining. .. After 3 washes, coverslips were counterstained with DAPI, then mounted with ProLong Diamond (ThermoFisher) antifade medium and examined under a confocal microscope (Zeiss LSM 700).

    Staining:

    Article Title: Kinin B1 Receptor Blockade Prevents Angiotensin II-induced Neuroinflammation and Oxidative Stress in Primary Hypothalamic Neurons.
    Article Snippet: For GFAP staining, an Alexa Fluor 488-labeled antibody (#53–9892-82, lot 1,914,350, ThermoFisher, 1:50 dilution) was used. .. Triple immunostaining was performed with specific validated antibodies for detection of AT1R (#AAR-011, lot AN2002, Alomone labs, 1:200 dilution) and B1R (#ABR-011, lot An-01, Alomone labs, 1:200 dilution) coupled with MAP2 and DAPI staining. .. After 3 washes, coverslips were counterstained with DAPI, then mounted with ProLong Diamond (ThermoFisher) antifade medium and examined under a confocal microscope (Zeiss LSM 700).

    Article Title: Kinin B1 Receptor Blockade Prevents Angiotensin II-induced Neuroinflammation and Oxidative Stress in Primary Hypothalamic Neurons
    Article Snippet: For GFAP staining, an Alexa Fluor 488 labeled antibody (#53-9892-82, lot 1,914,350, ThermoFisher, 1:50 dilution) was used. .. Triple immunostaining was performed with specific validated antibodies for detection of AT1R (#AAR-011, lot AN2002, Alomone labs, 1:200 dilution) and B1R (#ABR-011, lot An-01, Alomone labs, 1:200 dilution) coupled with MAP2 and DAPI staining. .. After 3 washes, coverslips were counterstained with DAPI, then mounted with ProLong Diamond (ThermoFisher) antifade medium and examined under a confocal microscope (Zeiss LSM 700).

    Incubation:

    Article Title: Targeting Kinin B1R Attenuates Hypertension Through AT1R-Dependent Mechanisms
    Article Snippet: .. Then sections were incubated with B1R (Alomone ABR-011 or LSBio LS-C54590, 1:250), AT1R (Alomone AAR-011, 1:250), vGLUT2 (Abcam ab216463, 1:500), cFOS (Cell signaling 2250S, 1:500), NeuN (Sigma MAB377, 1:500), TMEM119 (Synaptic Systems 400- 002, 1:500), PSD-95 (Invitrogen MA1-046, 1:500), Tyrosine hydroxylase (Sigma AB152, 1:500), GFAP (Thermo 14-9892-82), homer1 (synaptic systems 160-003, 1:500), synaptophysin1 (Synaptic systems 101-011, 1:500), IBA1 (Wako 011-27991, 1:1000), and HuC/HuD (Invitrogen A21271, 1:500) antibodies at 4°C overnight in 1xPBS containing 1% normal donkey serum and 0.1% Tween20. ..

    Article Title: Long COVID in K18-hACE2 mice causes persistent brain inflammation and cognitive impairment
    Article Snippet: .. Free floating brain sections were 384 washed with PBS and blocked with 5% donkey serum in 1 × PBS containing 0.2% Tween-20 for 1 h. They 385 were then incubated with appropriate primary antibodies: B1R (#ABR-011, lot An-01, Alomone labs, 386 1:500 dilution), IL-6 (12912S, lot 2, Cell Signaling Technologies, 1:500) TNF (11948S, lot 5, Cell Signaling 387 Technologies, 1:500), AT1R (#AAR-011, lot AAR011AN2002, Alomone labs, 1:500), NeuN (MAB377, lot 388 3808682, EMD Millipore, 1:500). ..

    Article Title: Emerging Role of Kinin B1 Receptor in Persistent Neuroinflammation and Neuropsychiatric Symptoms in Mice Following Recovery from SARS-CoV-2 Infection
    Article Snippet: .. Free-floating brain sections were washed with PBS and blocked with 5% donkey serum in 1 × PBS containing 0.2% Tween-20 for 1 h. They were then incubated with appropriate primary antibodies: B1R (#ABR-011, lot An-01, Alomone labs, Jerusalem, Israel; 1:500 dilution), IL-6 (12912S, lot 2, Cell Signaling Technologies, Beverly, MA, USA; 1:500) TNF (11948S, lot 5, Cell Signaling Technologies, Beverly, MA, USA; 1:500), AT1R (#AAR-011, lot AAR011AN2002, Alomone labs, Jerusalem, Israel; 1:500), NeuN (MAB377, lot 3808682, Millipore Sigma, Burlington, MA, USA; 1:500), SARS-CoV antiserum (NR-10362, BEI resources, Manassas, VA, USA; 1:500), and dsRNA (Ab01299-23.0, absolute antibody, Boston, MA, USA; 1:500). .. The next day, sections were washed with PBS + 0.2% Tween-20 and incubated with appropriate Alexa flour conjugated secondary antibodies (ThermoFisher, Hanover Park, IL, USA; 1:1000 dilution) for 1 h at room temperature, followed by DAPI nuclear stain.

    Article Title: Emerging Role of Kinin B1 Receptor in Persistent Neuroinflammation and Neuropsychiatric Symptoms in Mice Following Recovery from SARS-CoV-2 Infection.
    Article Snippet: .. Free-floating brain sections were washed with PBS and blocked with 5% donkey serum in 1×PBS containing 0.2% Tween-20 for 1 h. They were then incubated with appropriate primary antibodies: B1R (#ABR-011, lot An-01, Alomone labs, Jerusalem, Israel; 1:500 dilution), IL-6 (12912S, lot 2, Cell Signaling Technologies, Beverly, MA, USA; 1:500) TNF (11948S, lot 5, Cell Signaling Technologies, Beverly, MA, USA; 1:500), AT1R (#AAR-011, lot AAR011AN2002, Alomone labs, Jerusalem, Israel; 1:500), NeuN (MAB377, lot 3808682, Millipore Sigma, Burlington, MA, USA; 1:500), SARS-CoV antiserum (NR10362, BEI resources, Manassas, VA, USA; 1:500), and dsRNA (Ab01299-23.0, absolute antibody, Boston, MA, USA; 1:500). .. The next day, sections were washed with PBS + 0.2% Tween-20 and incubated with appropriate Alexa flour conjugated secondary antibodies (ThermoFisher, Hanover Park, IL, USA; 1:1000 dilution) for 1 h at room temperature, followed by DAPI nuclear stain.

    Article Title: Unique Expression of Angiotensin Type-2 Receptor in Sex-Specific Distribution of Myelinated Ah-Type Baroreceptor Neuron Contributing to Sex-Dimorphic Neurocontrol of Circulation
    Article Snippet: The sex difference in blood pressure (BP) has long been recognized between premenopausal women and agedmatched men.. Women are protected from most cardiovascular events compared with age-matched men before menopause, and postmenopausal women are at increased risk of cardiovascular complications compared with premenopausal women.. The pathophysiological mechanisms have been extensively explored, and increasing evidences have shown that the female hormone is one of the major mechanisms contributing to the above phenomena.

    Cell-Signaling:

    Article Title: Targeting Kinin B1R Attenuates Hypertension Through AT1R-Dependent Mechanisms
    Article Snippet: .. Then sections were incubated with B1R (Alomone ABR-011 or LSBio LS-C54590, 1:250), AT1R (Alomone AAR-011, 1:250), vGLUT2 (Abcam ab216463, 1:500), cFOS (Cell signaling 2250S, 1:500), NeuN (Sigma MAB377, 1:500), TMEM119 (Synaptic Systems 400- 002, 1:500), PSD-95 (Invitrogen MA1-046, 1:500), Tyrosine hydroxylase (Sigma AB152, 1:500), GFAP (Thermo 14-9892-82), homer1 (synaptic systems 160-003, 1:500), synaptophysin1 (Synaptic systems 101-011, 1:500), IBA1 (Wako 011-27991, 1:1000), and HuC/HuD (Invitrogen A21271, 1:500) antibodies at 4°C overnight in 1xPBS containing 1% normal donkey serum and 0.1% Tween20. ..

    Article Title: Long COVID in K18-hACE2 mice causes persistent brain inflammation and cognitive impairment
    Article Snippet: .. Free floating brain sections were 384 washed with PBS and blocked with 5% donkey serum in 1 × PBS containing 0.2% Tween-20 for 1 h. They 385 were then incubated with appropriate primary antibodies: B1R (#ABR-011, lot An-01, Alomone labs, 386 1:500 dilution), IL-6 (12912S, lot 2, Cell Signaling Technologies, 1:500) TNF (11948S, lot 5, Cell Signaling 387 Technologies, 1:500), AT1R (#AAR-011, lot AAR011AN2002, Alomone labs, 1:500), NeuN (MAB377, lot 388 3808682, EMD Millipore, 1:500). ..

    Article Title: Emerging Role of Kinin B1 Receptor in Persistent Neuroinflammation and Neuropsychiatric Symptoms in Mice Following Recovery from SARS-CoV-2 Infection
    Article Snippet: .. Free-floating brain sections were washed with PBS and blocked with 5% donkey serum in 1 × PBS containing 0.2% Tween-20 for 1 h. They were then incubated with appropriate primary antibodies: B1R (#ABR-011, lot An-01, Alomone labs, Jerusalem, Israel; 1:500 dilution), IL-6 (12912S, lot 2, Cell Signaling Technologies, Beverly, MA, USA; 1:500) TNF (11948S, lot 5, Cell Signaling Technologies, Beverly, MA, USA; 1:500), AT1R (#AAR-011, lot AAR011AN2002, Alomone labs, Jerusalem, Israel; 1:500), NeuN (MAB377, lot 3808682, Millipore Sigma, Burlington, MA, USA; 1:500), SARS-CoV antiserum (NR-10362, BEI resources, Manassas, VA, USA; 1:500), and dsRNA (Ab01299-23.0, absolute antibody, Boston, MA, USA; 1:500). .. The next day, sections were washed with PBS + 0.2% Tween-20 and incubated with appropriate Alexa flour conjugated secondary antibodies (ThermoFisher, Hanover Park, IL, USA; 1:1000 dilution) for 1 h at room temperature, followed by DAPI nuclear stain.

    Article Title: Emerging Role of Kinin B1 Receptor in Persistent Neuroinflammation and Neuropsychiatric Symptoms in Mice Following Recovery from SARS-CoV-2 Infection.
    Article Snippet: .. Free-floating brain sections were washed with PBS and blocked with 5% donkey serum in 1×PBS containing 0.2% Tween-20 for 1 h. They were then incubated with appropriate primary antibodies: B1R (#ABR-011, lot An-01, Alomone labs, Jerusalem, Israel; 1:500 dilution), IL-6 (12912S, lot 2, Cell Signaling Technologies, Beverly, MA, USA; 1:500) TNF (11948S, lot 5, Cell Signaling Technologies, Beverly, MA, USA; 1:500), AT1R (#AAR-011, lot AAR011AN2002, Alomone labs, Jerusalem, Israel; 1:500), NeuN (MAB377, lot 3808682, Millipore Sigma, Burlington, MA, USA; 1:500), SARS-CoV antiserum (NR10362, BEI resources, Manassas, VA, USA; 1:500), and dsRNA (Ab01299-23.0, absolute antibody, Boston, MA, USA; 1:500). .. The next day, sections were washed with PBS + 0.2% Tween-20 and incubated with appropriate Alexa flour conjugated secondary antibodies (ThermoFisher, Hanover Park, IL, USA; 1:1000 dilution) for 1 h at room temperature, followed by DAPI nuclear stain.

    Blocking Assay:

    Article Title: Unique Expression of Angiotensin Type-2 Receptor in Sex-Specific Distribution of Myelinated Ah-Type Baroreceptor Neuron Contributing to Sex-Dimorphic Neurocontrol of Circulation
    Article Snippet: The sex difference in blood pressure (BP) has long been recognized between premenopausal women and agedmatched men.. Women are protected from most cardiovascular events compared with age-matched men before menopause, and postmenopausal women are at increased risk of cardiovascular complications compared with premenopausal women.. The pathophysiological mechanisms have been extensively explored, and increasing evidences have shown that the female hormone is one of the major mechanisms contributing to the above phenomena.



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    A role for <t>AT1R-Gα12-Pyk2-Gab1-PKCθ</t> signaling axis in Ang II-induced ABCA1 dissociation from CSN5 and association with cullin3 leading to its ubiquitination and degradation. A, Quiescent RAW264.7 cells were treated with and without Ang II (1 μM) for the indicated time periods, cell extracts were prepared and analyzed by Western blotting for AT1R and β-actin levels using their specific antibodies and quantified. B, All the conditions are same as in panel A except that cell lysates were immunoprecipitated <t>with</t> <t>anti-AT1R</t> antibody and the immunocomplexes were analyzed by Western blotting for the indicated proteins. The input protein was immunoblotted for β-actin. C, Cell lysates in panel A were analyzed by Western blotting for the indicated proteins using their specific antibodies and quantified. D, Quiescent RAW264.7 cells were treated with and without Ang II (1 μM) in the presence or absence of losartan (10 μM) for 4 h, cell extracts prepared, immunoprecipitated with anti-ABCA1 antibody and the immunocomplexes were analyzed by Western blotting for the indicated proteins using their specific antibodies. The input protein was immunoblotted for β-actin. E and F, RAW264.7 cells that were transfected with the indicated siRNAs were quiesced, treated with and without Ang II (1 μM) for 4 h, cell extracts prepared, immunoprecipitated with anti-ABCA1 antibody and the immunocomplexes were analyzed by Western blotting for the indicated proteins and quantified. G, The cell extracts from panels D, E and F were analyzed by Western blotting for xanthine oxidase using its specific antibody and the blots were normalized for β-actin. The bar graphs represent Mean ± SD values of three biologically independent experiments. ∗p < 0.01 versus control or siControl; # p < 0.01 versus Ang II or siControl + Ang II.
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    The impact of RDN on the circadian expression of renal RAS components in various groups of rats ( n =8). (A) Representative images of ACE1, ACE2, <t>AT1R,</t> and MasR proteins, along with their quantitative analysis. (B) Angiotensin-converting enzyme 1 (ACE1), (C) Angiotensin-converting enzyme 2 (ACE2), (D) Angiotensin II type 1 receptor (AT1R), and (E) Angiotensin 1-7 receptor (MasR). Day: resting phase, Night: active phase. Compared to the resting phase (Day), a P < 0.05; in comparison to the overall WKY group, b P < 0.05; and compare to the overall Sham group, c P < 0.05.
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    Image Search Results


    A role for AT1R-Gα12-Pyk2-Gab1-PKCθ signaling axis in Ang II-induced ABCA1 dissociation from CSN5 and association with cullin3 leading to its ubiquitination and degradation. A, Quiescent RAW264.7 cells were treated with and without Ang II (1 μM) for the indicated time periods, cell extracts were prepared and analyzed by Western blotting for AT1R and β-actin levels using their specific antibodies and quantified. B, All the conditions are same as in panel A except that cell lysates were immunoprecipitated with anti-AT1R antibody and the immunocomplexes were analyzed by Western blotting for the indicated proteins. The input protein was immunoblotted for β-actin. C, Cell lysates in panel A were analyzed by Western blotting for the indicated proteins using their specific antibodies and quantified. D, Quiescent RAW264.7 cells were treated with and without Ang II (1 μM) in the presence or absence of losartan (10 μM) for 4 h, cell extracts prepared, immunoprecipitated with anti-ABCA1 antibody and the immunocomplexes were analyzed by Western blotting for the indicated proteins using their specific antibodies. The input protein was immunoblotted for β-actin. E and F, RAW264.7 cells that were transfected with the indicated siRNAs were quiesced, treated with and without Ang II (1 μM) for 4 h, cell extracts prepared, immunoprecipitated with anti-ABCA1 antibody and the immunocomplexes were analyzed by Western blotting for the indicated proteins and quantified. G, The cell extracts from panels D, E and F were analyzed by Western blotting for xanthine oxidase using its specific antibody and the blots were normalized for β-actin. The bar graphs represent Mean ± SD values of three biologically independent experiments. ∗p < 0.01 versus control or siControl; # p < 0.01 versus Ang II or siControl + Ang II.

    Journal: Redox Biology

    Article Title: ROS disrupt COP9 signalosome-mediated ABCA1 protection and trigger its ubiquitination and degradation by cullin3 inhibiting cholesterol efflux and promoting foam cell formation in response to GPCR agonists

    doi: 10.1016/j.redox.2026.104095

    Figure Lengend Snippet: A role for AT1R-Gα12-Pyk2-Gab1-PKCθ signaling axis in Ang II-induced ABCA1 dissociation from CSN5 and association with cullin3 leading to its ubiquitination and degradation. A, Quiescent RAW264.7 cells were treated with and without Ang II (1 μM) for the indicated time periods, cell extracts were prepared and analyzed by Western blotting for AT1R and β-actin levels using their specific antibodies and quantified. B, All the conditions are same as in panel A except that cell lysates were immunoprecipitated with anti-AT1R antibody and the immunocomplexes were analyzed by Western blotting for the indicated proteins. The input protein was immunoblotted for β-actin. C, Cell lysates in panel A were analyzed by Western blotting for the indicated proteins using their specific antibodies and quantified. D, Quiescent RAW264.7 cells were treated with and without Ang II (1 μM) in the presence or absence of losartan (10 μM) for 4 h, cell extracts prepared, immunoprecipitated with anti-ABCA1 antibody and the immunocomplexes were analyzed by Western blotting for the indicated proteins using their specific antibodies. The input protein was immunoblotted for β-actin. E and F, RAW264.7 cells that were transfected with the indicated siRNAs were quiesced, treated with and without Ang II (1 μM) for 4 h, cell extracts prepared, immunoprecipitated with anti-ABCA1 antibody and the immunocomplexes were analyzed by Western blotting for the indicated proteins and quantified. G, The cell extracts from panels D, E and F were analyzed by Western blotting for xanthine oxidase using its specific antibody and the blots were normalized for β-actin. The bar graphs represent Mean ± SD values of three biologically independent experiments. ∗p < 0.01 versus control or siControl; # p < 0.01 versus Ang II or siControl + Ang II.

    Article Snippet: Anti-β-actin (sc-47778), anti-ABCG1 (sc-20795), anti-AT1R (sc-515884), anti-Gα12 (sc-409), anti-Gα13 (sc-293424), anti-Gab1 (sc-6292), anti-Par1 (sc-13503), anti-SRB1 (sc-67099), and anti-XO (sc-398548) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

    Techniques: Ubiquitin Proteomics, Western Blot, Immunoprecipitation, Transfection, Control

    The impact of RDN on the circadian expression of renal RAS components in various groups of rats ( n =8). (A) Representative images of ACE1, ACE2, AT1R, and MasR proteins, along with their quantitative analysis. (B) Angiotensin-converting enzyme 1 (ACE1), (C) Angiotensin-converting enzyme 2 (ACE2), (D) Angiotensin II type 1 receptor (AT1R), and (E) Angiotensin 1-7 receptor (MasR). Day: resting phase, Night: active phase. Compared to the resting phase (Day), a P < 0.05; in comparison to the overall WKY group, b P < 0.05; and compare to the overall Sham group, c P < 0.05.

    Journal: Frontiers in Cardiovascular Medicine

    Article Title: Impact of renal denervation on circadian variations of blood pressure and clock gene expression in spontaneously hypertensive rats

    doi: 10.3389/fcvm.2026.1739153

    Figure Lengend Snippet: The impact of RDN on the circadian expression of renal RAS components in various groups of rats ( n =8). (A) Representative images of ACE1, ACE2, AT1R, and MasR proteins, along with their quantitative analysis. (B) Angiotensin-converting enzyme 1 (ACE1), (C) Angiotensin-converting enzyme 2 (ACE2), (D) Angiotensin II type 1 receptor (AT1R), and (E) Angiotensin 1-7 receptor (MasR). Day: resting phase, Night: active phase. Compared to the resting phase (Day), a P < 0.05; in comparison to the overall WKY group, b P < 0.05; and compare to the overall Sham group, c P < 0.05.

    Article Snippet: Antibodies and dilutions were as follows: ACE1, 1:1000 dilution (A11357, ABclonal, China); ACE2, 1:1000 dilution (A12737, ABclonal, China); AT1R, 1:1000 dilution (25343-1-AP, Proteintech, China) and MasR, 1:800 dilution (sc-390453, Santa Cruz, USA).

    Techniques: Expressing, Comparison